Multimedia Gallery

[KESM specifications]

Knife-Edge Scanning Microscope

Click on the images to view the full-sized version.

 

A full view of the Knife-Edge Scanning Microscope. Note the upgraded knife assembly on the right (also see the close-up shot right below).
[AVI Movie] (10.4MB) [AVI Movie: different encoding] (2.6MB)
NEW 7/18/2005
Redesigned knife assembly and light source (right) for improved chatter resistence.
NEW 7/18/2005
Cutting session: Microscope objective (left) and the diamond knife (right) in place for cutting. The specimen (block in the center) and the knife are fully submerged.
[AVI Movie] (9.9MB) [AVI Movie: different encoding] (1.57MB)
High-res version:[AVI Movie] (30MB) [AVI Movie: different encoding] (5.71MB)
NEW 7/18/2005
An illustration of how the diamond knife provides illumination. The objective is shown on the left and the white light illumination and the knife assembly are shown to the right.
An illustration of how the diamond knife cuts the tissue. The objective is shown on the left and the light source and the knife assembly are shown to the right.
The tip of the diamond knife as seen through the observation eyepiece. The translucent tissue just cut off is shown on top of the diamond knife (left). The osmium-stained mouse brain can be seen below to the right. Note that this movie is shown in real time!
[Low-res AVI Movie] (17.9MB) [Low-res AVI Movie: different encoding] (4.26MB)
[High-res AVI Movie] (37MB) [High-res AVI Movie: different encoding] (6.5MB)
NEW 7/18/2005
Closeup of KESM optics and diamond knife assembly. The green glowing part is the diamond knife, and the black blob below is the osmium-stained mouse brain embedded in LR-White.
NEW 7/18/2005
Closeup view of the submerged mouse brain (osmium stained) with the light turned on on the diamond knife (the green glowing part).
NEW 7/18/2005