1. Set ISPALIGN_DIR in src/default.h to the absolute path in which the ispalign directory is located. 2. Type ./install to install ispalign. 3. The executable file ispalign is in bin. Either move it to a directory on the search path or add the directory bin to the search path. 4. Execute ispalign with the command bin/ispalign 1aboA_ref1.pep where 1aboA_ref1.pep is a fasta input file included as an example. Core dumps from this step can be ignored. The alignment will still be produced correctly. The current version of ispalign accepts one argument specifying the input file in fasta format. The output alignment will be put into a file with extension .aln added to the filename. More information on ispalign is available at http://faculty.cs.tamu.edu/shsze.